Hi. I have virtually all of my Mp3s at 320 kbps. I also wondered if I would notice any difference encoding them at apple lossless quality.
I tested about a dozen different songs. Encoding them at both 320 kbps and apple lossless. Then added them to a playlist, shuffled them and listenend.
I personally could not notice any difference. I think it depends on alot of variables. ie what heaphones, speakers etc you are using. And without overlooking the obvious, how good your own ears are. Some people will have better hearing than others, and will notice differences.
My advice is to try something along the lines of what I did and decide for yourself if it is worth switching to apple lossless.